The qRT-PCR in A Nasal Swab Leprosy Patient’s at Dr. M. Djamil Padang Hospital
Abstract
Leprosy, caused by Mycobacterium leprae, in 2016, Indonesia reported 16,826 new leprosy cases, with a prevalence rate of 0.71 per 10,000 individuals and a new case detection rate of 6.5 per 10,000 individuals. This study describes the result of a quantitative real-time polymerase chain reaction (qRT-PCR) in the nasal mucosa smear of a leprosy patient at the Dermatology and Venereology Polyclinic of Dr. M. Djamil Padang Hospital. This study adopts an observational approach with a width cut design, specifically employing a cross-sectional study methodology on leprosy patient at the Dermatology and Venereology Polyclinic of Dr. M. Djamil Padang Hospital, Indonesia between February 2023 until February 2024. The qRT-PCR quantity value of nasal mucous discharge is analyzed by a real-time PCR machine. The provided data presents characteristics of a sample population (N = 36) with leprosy. The majority of the leprosy-affected individuals in this study are between 8 and 68 years old, with a median age of 34. According to the World Health Organization (WHO) classification indicates that 83.33% of cases are multibacillary (MB). A combination RLEP/ qRT-PCR assay was developed to enable laboratory-based care and follow-up of leprosy patients attending our outpatient polyclinic in M. Djamil, Padang. The assay offers a sensitive and precise method for assessing the bacterial load and viability of M. leprae from nasal swab samples. It can be used for treatment response monitoring and early diagnosis.
References
Adhin, A. (2020). Desain Sekuen Primer Dan Probe Quantitative Polymerase Chain Reaction Gen Spesifik RLEP Mycobacterium Leprae. Jurnal Analis Medika Biosains (JAMBS), 7(1), 1. https://doi.org/10.32807/jambs.v7i1.159
Alferdi, A. (2022). Resiliensi Hospitality Yesus terhadap Orang Kusta dan Implementasinya bagi Orang Percaya di Masa Pandemi Covid-19. KENOSIS: Jurnal Kajian Teologi, 8(1), 41–54. https://doi.org/10.37196/kenosis.v8i1.384
Dinas Kesehatan Padang Pariaman. (2021). Laporan Tahunan Puskesmas Pauh Kambar.
Goldsmith, L. A., Katz, S. I., & Gilchrest, B. A. (2019). Fitzpatrick_s Dermatology in General Medicine. 2288.
Gupta, R., Kar, H. K., & Bharadwaj, M. (2012). Revalidation of various clinical criteria for the classification of leprosy--a clinic-pathological study. Leprosy Review, 83(4), 354–362.
Jadhav, R. S., Kamble, R. R., Shinde, V. S., Edward, S., & Edward, V. K. (2005). Use of reverse transcription polymerase chain reaction for the detection of Mycobacterium leprae in the slit-skin smears of leprosy patients. Indian Journal of Leprosy, 77(2), 116–127.
Jasani, J., & Maru, A. (2022). A clinico-histopathological analysis and bacillary index in a study of skin biopsy of leprosy. International Journal of Health Sciences, 6(June), 11014–11023. https://doi.org/10.53730/ijhs.v6ns5.11273
Jha, S., Dangol, A., Shakya, S., & Jha, B. (2016). Clinico-bacterial correlation of bacterial index in Hansen’s disease. Journal of Pathology of Nepal, 6(12), 998–1000. https://doi.org/10.3126/jpn.v6i12.16285
Jiang, H., Shi, Y., Chokkakula, S., Zhang, W., Long, S., Wang, Z., Kong, W., Long, H., Wu, L., Hu, L., Yao, Q., & Wang, H. (2022). Utility of Multi-target Nested PCR and ELISPOT Assays for the Detection of Paucibacillary Leprosy: A Possible Conclusion of Clinical Laboratory Misdiagnosis. Frontiers in Cellular and Infection Microbiology, 12(April). https://doi.org/10.3389/fcimb.2022.814413
Kurniatillah, N., Hayat, F., & Lusinto, W. H. (2022). Situasi Penyakit Kusta Di Kabupaten Serang Tahun 2020. Journal of Baja Health Science, 2(02), 148–157. https://doi.org/10.47080/joubahs.v2i02.2194
Manta, F. S. N., Barbieri, R. R., Moreira, S. J. M., Santos, P. T. S., Nery, J. A. C., Duppre, N. C., Sales, A. M., Pacheco, A. G., Hacker, M. A., Machado, A. M., Sarno, E. N., & Moraes, M. O. (2019). Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018. Scientific Reports, 9(1), 1–8. https://doi.org/10.1038/s41598-019-52640-5
Martins, R. J., Carloni, M. E. O. G., Moimaz, S. A. S., Garbin, C. A. S., & Garbin, A. J. Í. (2016). Sociodemographic and epidemiological profile of leprosy patients in an endemic region in Brazil. Revista Da Sociedade Brasileira de Medicina Tropical, 49(6), 777–780. https://doi.org/10.1590/0037-8682-0069-2016
Morgado de Abreu, M. A. M., Roselino, A. M., Enokihara, M., Nonogaki, S., Prestes-Carneiro, L. E., Weckx, L. L. M., & Alchorne, M. M. A. (2014). Mycobacterium leprae is identified in the oral mucosa from paucibacillary and multibacillary leprosy patients. Clinical Microbiology and Infection : The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 20(1), 59–64. https://doi.org/10.1111/1469-0691.12190
Muddebihal, A., Sardana, K., Khurana, A., Sachdeva, S., Singh, I., Ahuja, M., & Sharma, R. (2023). A prospective case control study of resistance to rifampicin, dapsone and ofloxacin in Type 1 and Type 2 leprosy reactions and the therapeutic impact of modified treatment regimen on reactions. In Journal of the European Academy of Dermatology and Venereology : JEADV (Vol. 37, Issue 2, pp. e149–e151). https://doi.org/10.1111/jdv.18709
Naaz, Farah; Mohanty, Partha Sarathi; Bansal, Avi Kumar; Kumar, Dilip; Gupta, U. D. (2017). Challenges Beyond Elimination in Leprosy. International Journal of Mycobacteriology, 6(3), 222–228. https://doi.org/10.4103/ijmy.ijmy_70_17
Nath, I., Saini, C., & Valluri, V. L. (2015). Immunology of leprosy and diagnostic challenges. Clinics in Dermatology, 33(1), 90–98. https://doi.org/10.1016/j.clindermatol.2014.07.005
Patrocínio, L. G., Goulart, I. M. B., Goulart, L. R., Patrocínio, J. A., Ferreira, F. R., & Fleury, R. N. (2005). Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction. FEMS Immunology and Medical Microbiology, 44(3), 311–316. https://doi.org/10.1016/j.femsim.2005.01.002
Pranata, M., Nugrahaini, A. R. D. N., & Fajariah, N. (2022). Characteristics and Therapy in Leprosy Patients at The Semarang City of Tugurejo Hospital. Medical Sains, 7(4), 943–950.
Reis, E. M., Araujo, S., Lobato, J., Neves, A. F., Costa, A. V, Gonçalves, M. A., Goulart, L. R., & Goulart, I. M. B. (2014). Mycobacterium leprae DNA in peripheral blood may indicate a bacilli migration route and high-risk for leprosy onset. Clinical Microbiology and Infection : The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 20(5), 447–452. https://doi.org/10.1111/1469-0691.12349
Republik Indonesia, K. kesehatan. (2020). Buku Pedoman Pelayanan Kusta.
RI, K. K. (2018). Pusat data dan Informasi Kementerian Kesehatan RI. Jakarta.
Siswanto, T. Asrianti, and D. M. (2020). Neglected Tropical Disease Kusta (Epidemiologi Aplikatif). Mulawarman Univ. Press, 1–65.
Suneetha, S., Arunthathi, S., Kurian, N., & Chacko, C. J. (n.d.). Histological changes in the nerve, skin and nasal mucosa of patients with primary neuritic leprosy. Acta Leprologica, 12(1), 11–18.
Tatipally, S., Srikantam, A., & Kasetty, S. (2018). Polymerase Chain Reaction (PCR) as a Potential Point of Care Laboratory Test for Leprosy Diagnosis-A Systematic Review. Tropical Medicine and Infectious Disease, 3(4). https://doi.org/10.3390/tropicalmed3040107
Viljoen, G., Nel, L., & Crowther, J. (2005). Molecular Diagnostic PCR Handbook.
WHO. (2023). Leprosy. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/leprosy
Wirayasa, A. (2022). Nasal Swab Multiplex PCR (MPCR) sebagai Metode Diagnosis Dini pada Narakontak Serumah Penyandang Lepra. Jurnal Sosial Dan Sains, 2(5), 638–647. https://doi.org/10.59188/jurnalsosains.v2i5.398
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